PowerPoint Presentation - Cancer Cell Culturing and CytotoxicityAssays for Anticancer Screening at City of Hope

CellMaintenance and Technique
nCell growth and passage
¨Tissue culture medium
¨Antibiotics
¨Trypsin, serum, L-glutamine
¨Cryopreservation and storage
nCell Counting
¨Hemocytometer
¨Coulter counter
nSafety and technique
An example of removing Media from a T25 flask
Masters, J., Stacey, G.(2007). Changing medium and passaging cell lines. Nature Protocols 2: 2276-2284.