oCellswere grown in expansion medium
oAlpha-MinimalEssential Medium supplemented with Fetal bovine serum, penicillin, streptomycin,L-glutamine, and ascorbic acid 2- phosphate.
oDifferentiationwas induced by changing medium conditions.
oExpansionmedium was supplemented with dexamethasone, sodium pyruvate, hepes, and inorganic phosphate to inducedifferentiation to bone.
oDifferentiationwas carried out for 28 days
oRNA wascollected at days 2, 4, 7, 14, 21, and 28
oExpressionof the genes listed above along with bone marker genes was measured by real timeRT PCR