Spring 2011 Biomedical Seminar Series
Friday, December 2, 2011
Splicing Viruses and Single Molecules into Electronics
Dr. Gregory Weiss
Department of Chemistry, Molecular Biology and Biochemistry
Gregory A. Weiss1,2, Jessica Arter2, Issa Moody1,Keith Donavan2, Yongki Choi3, Patrick Sims3, Phil Collins3, Reginald M. Penner1 Departments of Chemistry1, Molecular Biology and Biochemistry2, Physics3 University of California, Irvine Irvine, CA 92697-2025 USA
Our laboratory explores the intersection of biology and electronics. For biosensor development, we collaborate with electrochemists Prof. Reg Penner and co-workers to directly correlate changes in electrical impedance with molecular recognition. M13 bacteriophage engineered for cancer biomarker binding allow detection of the biomarkers, and are incorporated by electrodeposition into nanowires and films composed of an electrically conductive organic polymer (PEDOT) (1). Together with our collaborator, physicists Prof. Phil Collins and co-workers, we have demonstrated conductance-controlled introduction of a single, carboxylate handle onto the sidewall of a nanotube (2). Using the electronic signature of the resultant nanocircuit, the single protein can be examined in real-time during protein unfolding, folding, binding, and, where applicable, catalysis. Results from examining a single lysozyme tethered to the nanocircuit extend our understanding of the enzymeÂs dynamics to the enzymeÂs responses to substrate branching, changes to the pH, and other conformational changes critical for catalysis. 1. Arter, J. A., D. K. Taggart, T. M. McIntire, R. M. Penner, and G. A. Weiss. 2010. Virus-PEDOT Nanowires for Biosensing. Nano Lett. 10: 4858-4862. 2. Goldsmith, B. R., J. G. Coroneus, V. R. Khalap, A. A. Kane, G. A. Weiss, and P. G. Collins. 2007. Conductance-controlled point functionalization of single-walled carbon nanotubes. Science 315: 77-81.