outline_expanded.htm

Analysis  of Id-1 and Twist-1 Regulation in Bone Development
Anna E. Mu–oz
Cal State University, Los Angeles-City of Hope Cancer Collaborative
April  7, 2008

Outline
Introduction
Helix-Loop-Helix proteins
Id-1 and Twist-1
Human stem cells
Study model system
Significance of Study
Cell line preliminary results
Collaborative research project
Project overview
Acknowledgements

Helix Loop Helix Proteins
Various helix-loop-helix (HLH) proteins play a key role in the regulation of cellular growth and differentiation
Basic HLH, bHLH, proteins include a basic DNA binding domain
MyoD (directs muscle development) and TWIST-1
HLH proteins lack the basic domain
Id proteins do not bind DNA

Id-1
Twist-1
Slide 6
Stem Cells
Mesenchymal Stem Cells
StudyÕs Model System
Normal human cells undergo a limited number of cell divisions in culture
Enter senescence, a non-dividing state
Telomere shortening has been linked to cellular senescence
Retroviral transduction of human telomerase reverse transcriptase (hTERT)
Maintains telomere length
Extends life span
Dr. GlackinÕs lab at COH created a human fetal mesenchymal stem cell line that has been immortalized by the hTERT gene, hfMSC-SK-hTERT cell line

Significance of Study


CELL LINE PRELIMINARY RESULTS
Experimental Methods
Experimental Methods
Experimental Methods
MSC differentiation to bone
Twist-1 and Id-1 Expression in Osteogenic Differentiation of MSCs
CANCER COLLABORATIVE RESEARCH PROJECT
Research Goals
Id-1 and Twist-1 Regulation in MSC-hTERT line
Human Id-1 and Twist-1 promoter constructs
Acknowledgements
CSULA-COH Cancer Collaborative Program
NIH grant
Dr. Sharp, Cal State LA
Laura Martinez, Sharp Lab
Dr. Glackin, City of Hope
Shan Li, Glackin Lab
Joyce Ho, Cal State LA Collaborating student

"Thank You"