October 22, 2004
Titile: Regulation of a virulence associated acid response
in Mycobacterium tuberculosis"
Abstract:Tuberculosis exacts an enormous burden in morbidity and mortality on the global population. With the advent of the HIV epidemic and multiple drug resistances, disease due to tuberculosis has increased leading to a need for research into the basic mechanisms of pathogenesis. When M. tuberculosis invades a host it infects human macrophages and remains within the phagosome which may acidify to pH 6.0. M. tuberculosis can also be found within host granulomas which have been shown to have an acidic pH. The promoter region of the M. tuberculosis gene lipF has been identified to be upregulated in response to acidic pH. Within this same gene locus, a divergently transcribed gene, Rv3488 has been identified to have homology to transcriptional regulators and has been shown to bind to plipF. We hope to characterize the acid response in Mycobacterium tuberculosis and provide the ground work for the eventual identification of a general mechanism by which M. tuberculosis can resist environmental stresses such as acidic stress to persist in the host.
Saviola, B., S., Bishai, W., Isolation of Acid Induced Genes of Mycobacterium tuberculosis using Recombinase-based in vivo Expression Technology. Infection and Immunity, Vol 71, No 3, 1379-1388